The principle of this method was to screen the pharmacological activity of ten prepared
polyphyto formulations by using highthroughput screening method for their antihyperlipidemic
activity. The study was performed in three stages using single animal, two animals and three animals
in a group. The data produced with single animal is compared with the average data shown by two and
three experimental animals and further with six animals (one way ANOVA) for the statistical
significance and was duly reported. R2 value was determined by Least squares linear regression
analysis to interpret as the proportion of the variance in the dependent variable that is predictable from the independent
variable. Test formulations were given at 50mg/kg, body weight through p.o. route daily for 14 days in experimental
animals induced with hyperlipidemia by injecting Triton X-100 at 100 mg/kg BW i/p and observed for 48 hrs for the
development of hyperlipidemia. Two best formulations namely PHF-21 and PHF-26 were selected by highthroughput
screening and results repeated in six animals in a group were found to be statically similar.
Keywords: Antihyperlipidemic, ANOVA, highthroughput, linear regression, Triton X-100.
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