Repeated dose Toxicity Study in Rats of the Formulation Containing Metformin and Sitagliptin using Microwave Assisted Acrylamide Grafted Cassia tora as Polymer

Author(s): Shubhasis Dan, Nilendra Chatterjee, Pallab Mandal, Anjan Das, Balaram Ghosh, Tapan Kumar Pal.

Journal Name: The Natural Products Journal

Volume 6 , Issue 2 , 2016

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Graphical Abstract:


Usage of excipients from natural sources is now the most attractive research areas. Most of the synthetic polymers used in the formulation are detrimental to some extent for the human physiological system. Herbal substitute will be the best choice to minimize long term adverse effect. The purposes of repeated dose toxicity testing are to obtain information about biologic activity of chemicals. In the present study, repeated oral toxicity study of formulation containing metformin 1000mg and sitagliptin 50mg in Swiss albino rats was studied. Microwave assisted acrylamide grafted C. tora is used as a polymer in the formulation.

Rats of both sexes from control and different dose groups (100, 200 and 400 mg/Kg body wt.) exhibited normal body weight gain throughout dosing period. Study protocol was prior approved by animal ethics committee. Combination treatment for 28 days, formulation did not show any significant features of haematological, behavioural, hepatotoxicity and nephrotoxicity in rats. Therefore it can be concluded that the combination using the grafted polymer is safe in these repeated doses in rats.

Keywords: Metformin, sitagliptin, toxicity, Grafted Cassia tora, swiss albino rats.

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Article Details

Year: 2016
Page: [152 - 160]
Pages: 9
DOI: 10.2174/2210315506666160503163018

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PDF: 18