Gymnema sylvestre R.Br. is an important medicinal plant traditionally used
as an antidiabetic agent. Excessive use of the plant has resulted in its mass depletion
from the natural habitat. In the present work, flavonoids were identified from the plant
parts and callus culture. Different colored callus was obtained using various concentrations
of hormones. The flavonoids (quercetin and kaempferol) were identified using
chromatographic and spectral studies. Total flavonoid content was found to be more
in aerial parts as compared to the callus tissue. However, antioxidant potential, in
terms of DPPH scavenging activity, was more in callus as compared to the leaves.
Keywords: Mass depletion, tissue culture, chromatographic, phenolic, DPPH.
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