Quantitative Proteomics with Isotope Dilution Analysis: Principles and Applications

Author(s): Kuanysh Kabytaev, Alexander Stoyanov.

Journal Name: Current Proteomics

Volume 13 , Issue 1 , 2016

Become EABM
Become Reviewer

Abstract:

Isotope dilution mass spectrometry (IDMS) is based on a simple and straightforward idea of using an isotope labeled compound as an internal standard for quantitation purposes. IDMS now competes with routine quantitative methods such as immunoassays (ELISA, RIA) due to its high precision, accuracy and robustness. The isotope dilution concept allows for protein quantitation and has been showing great promise in clinical application in the last decade. The present review contains a short description of the current status of proteomics and principles of absolute quantitative methodologies; it focuses on some mass spectrometric techniques, sample preparation and recent examples of IDMS applications for peptide and protein quantitation in clinical chemistry.

Keywords: Isotope dilution analysis, multi-dimensional separations, proteomics.

Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 13
ISSUE: 1
Year: 2016
Page: [61 - 67]
Pages: 7
DOI: 10.2174/1570164613666160413121219

Article Metrics

PDF: 20
HTML: 4
EPUB: 2
PRC: 1