Background: Epinephrine (EP), ascorbic acid (AA) and tyrosine (TY) usually coexist together
in human blood and are considered as important molecules for physiological processes in human metabolism.
Thus, to determine these compounds in biological fluids, we need to develop sensitive, simple and
Method: The simultaneous spectrophotometric determination of AA, EP and TY, at pH=7.0 using multivariate
calibration techniques such as principal component regression (PCR), partial least squares (PLS)
and their first derivative methods have been carried out.
Results: Satisfactory results were achieved using first derivative-partial least squares. After optimization
of all variables, the linear ranges for AA, EP and TY were 0.8-35.0, 1.0-72.01 and 1.0-145.0 µgmL-1,
respectively. The detection limits for AA, EP and TY obtained 0.1, 0.35 and 0.28 µgmL-1, respectively.
Principal component (NPC) for AA, EP and TY were 2, 3 and 3. Prediction error sum of squares (PRESS)
for AA, EP and TY were 2.37, 0.91 and 2.57, respectively. The root mean square errors of Prediction
(RMSEP) for AA, EP and TY were 0.35, 0.16 and 0.60 respectively.
Conclusion: Good agreement between prediction and actual concentrations clearly indicates the utilization
of this procedure for the simultaneous determination of AA, EP and TY in serum, plasma and urine
samples and also for the determination of AA in vitamin c tablet and EP in epinephrine injection.