Gene electrotransfer is an effective approach for delivering plasmid DNA to a variety of tissues.
Delivery of molecules with electric pulses requires control of the electrical parameters to achieve
effective delivery. Since discomfort or tissue damage may occur with high applied voltage, the reduction
of the applied voltage while achieving the desired expression may be an important improvement.
One possible approach is to combine electrotransfer with exogenously applied heat. Previous work
performed in vitro demonstrated that increasing temperature before pulsing can enhance gene expression
and made it possible to reduce electric fields while maintaining expression levels. In the study reported here, this
combination was evaluated in vivo using a novel electrode device designed with an inserted laser for application of heat.
The results obtained in this study demonstrated that increased temperature during electrotransfer increased expression or
maintained expression with a reduction in applied voltage. With further optimization this approach may provide the basis
for both a novel method and a novel instrument that may greatly enhance translation of gene electrotransfer.
Keywords: Gene electrotransfer, Skin, Gene therapy, Heat, Electroporation.
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