Background: The waste material from the preparation of propolis extracts is a potential
natural compound for application in pharmaceutical and medicine nanostructured products. Ascorbic
acid is an excellent antioxidant and an important cofactor of several physiological and biochemical
Objective: The aim of this study was to develop and characterize nanoparticles containing L-ascorbic
acid prepared with propolis byproduct.
Method: Nanoparticle's physicochemical characteristics (surface morphology, particle size, zeta potential,
and entrapment efficiency), antioxidant activity, in vitro release profile, and in vitro cytotoxicity
Results: Nanoparticles showed to be spherical, with agglomeration, mean diameter between 110.93
and 480.59 nm, zeta potential near zero and good entrapment efficiency. Antioxidant activity of
L-ascorbic acid increased when nanoencapsulated and the drug release was prolonged, controlled
mainly by the phenomenon of relaxation of polymer chains and dependent of propolis residue concentration.
The application of technology provided a reduction in the level of cytotoxicity of L-ascorbic
acid, and the nanoparticles showed a protective effect on macrophages.