Chemical stability of anthocyanins in aqueous solutions has been one of the major drawbacks
for health applications. Complexation of anthocyanins formed by aqueous extracts of dried cobs
of Zea mays L. ceritina Kulesh. (CC) and blue petals of Clitoria ternatea L. (CT) was challenged by
thermal accelerated stability testing of total anthocyanins by pH differential method, with in vitro antiinflammatory
and anti-candida screening tests. Decomposition of total anthocyanins of the anthocyanin complex (AC),
CC and CT in aqueous conditions follows first-order kinetic with derived activation energies of 53.3, 18.0, 34.4 kJ/mol,
respectively, indicating higher thermal tolerance by complexation. AC, at concentrations up to 2.5 mg/mL, was not cytotoxic
to the exposed human gingival epithelial cells (HGEPp0.5). At 0.1 µg/mL, AC recovers cellular proteins and nucleic
acids of TNF-alpha induced inflamed HGEPp0.5 cells, detected by FTIR spectroscopy and principle component analysis
(PCA). Anti-candida activity of AC was determined at 80 and 160 µg/mL as the minimum inhibitory and minimum fungicidal
concentrations, respectively. It is concluded that complexation improved chemical stability with anti-inflammatory
and anti-candida activities of the anthocyanins in an oral epithelial cell line.
Keywords: Anthocyanin complex, anti-candida, anti-inflammation, human gingival epithelial cells, stability, TNF-alpha.
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