Perspectives on Systematic Analyses of Gene Function in Arabidopsis thaliana: New Tools, Topics and Trends
Pp. 143-172 (30)
Cordelia Bolle, Anja Schneider and Dario Leister
Since the sequencing of the nuclear genome of Arabidopsis thaliana more
than ten years ago, various large-scale analyses of gene function have been performed
in this model species. In particular, the availability of collections of lines harbouring
random T-DNA or transposon insertions, which include mutants for almost all of the
~28,000 A. thaliana genes, has been crucial for the success of forward and reverse
genetic approaches. In the foreseeable future, genome-wide phenotypic data from
mutant analyses will become available for Arabidopsis, and will stimulate a flood of
novel in-depth gene-function analyses. In this review, we consider the present status of
resources and concepts for systematic studies of gene function in A. thaliana. Current
perspectives on the utility of loss-of-function and gain-of-function mutants will be
discussed in light of the genetic and functional redundancy of many A. thaliana genes.
Arabidopsis thaliana, EMS, forward genetics, gain of function, loss of
function, mutant, mutation, phenotype, reverse genetics, transposon, T-DNA.
Lehrstuhl für Molekularbiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, Großhaderner Str. 2, D-82152 Planegg- Martinsried, Germany.