Advances in Genome Science

Volume: 4

Genes in Health and Disease

Indexed in: EBSCO, Ulrich's Periodicals Directory

Genome science or genomics is essential to advancing knowledge in the fields of biology and medicine. Specifically, researchers learn about the molecular biology behind genetic expression in living ...
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Perspectives on Systematic Analyses of Gene Function in Arabidopsis thaliana: New Tools, Topics and Trends

Pp. 143-172 (30)

Cordelia Bolle, Anja Schneider and Dario Leister

Abstract

Since the sequencing of the nuclear genome of Arabidopsis thaliana more than ten years ago, various large-scale analyses of gene function have been performed in this model species. In particular, the availability of collections of lines harbouring random T-DNA or transposon insertions, which include mutants for almost all of the ~28,000 A. thaliana genes, has been crucial for the success of forward and reverse genetic approaches. In the foreseeable future, genome-wide phenotypic data from mutant analyses will become available for Arabidopsis, and will stimulate a flood of novel in-depth gene-function analyses. In this review, we consider the present status of resources and concepts for systematic studies of gene function in A. thaliana. Current perspectives on the utility of loss-of-function and gain-of-function mutants will be discussed in light of the genetic and functional redundancy of many A. thaliana genes.

Keywords:

Arabidopsis thaliana, EMS, forward genetics, gain of function, loss of function, mutant, mutation, phenotype, reverse genetics, transposon, T-DNA.

Affiliation:

Lehrstuhl für Molekularbiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, Großhaderner Str. 2, D-82152 Planegg- Martinsried, Germany.