Mycology: Current and Future Developments

Fungal Biotechnology for Biofuel Production

Indexed in: EBSCO.

Mycology: Current and Future Developments is a book series that brings together the latest contributions to research on the biology, genetics, and industrial use of fungi. Each book chapter is ...
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Protein Engineering Strategies to Improve Efficiency in Biomass Degradation

Pp. 202-221 (20)

Lucas F. Ribeiro, Tina Xiong, Pricila Hauk and Liliane F.C. Ribeiro

Abstract

Protein engineering has become the most important tool for enabling industrial application of biocatalysts. Advances in structure-guided methods and novel techniques for directed evolution and high-throughput screening have facilitated the design of enzymes with improved properties such as functional expression, stability, and catalytic activity. The utilization of lignocellulosic biomass for biofuels production is attractive because it is environmentally sustainable. However, commercialization of biomass biofuels requires efficient bioconversion of cellulosic material to sugar, which is largely hampered by biomass recalcitrance. In this chapter, we present the recent application of protein engineering techniques for maximizing the efficiencies of biocatalysts for biofuel production.

Keywords:

Protein engineering, enzyme adaptation, semi-rational design, library construction, site-directed mutagenesis, screening, biomass.

Affiliation:

Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.