Prostate cancer (PCa) is the most frequently diagnosed cancer and the second most common
cause of cancer related mortality in United States male population. ScFv fragments have different
usefulness. For example they have small size, high perfusion rate, high yield of production and are
non-immunogenic, thus they can be used for therapeutic purposes. In this project we used a synthetic
human ScFv library for isolation of ScFv monoclonal antibodies against prostate specific membrane
For this purpose, after five rounds of cell-panning, and also five rounds of antigen-panning with rPSMA specific anti-
PSMA ScFv-phage particles were isolated. Phages with high affinity toward PSMA were selected and used for further
Specificity and affinity of both ScFv to PSMA and LnCaP cell line examined by ELISA. Recombinant ScFv antibody isolated
from cell-panning had higher specificity and affinity for both the antigen and LNCaP cell line.
Our result demonstrated that ScFv antibody obtained by cell-panning can target PSMA antigen and cell lines.