Solid phase screenings of one bead one compound (OBOC) libraries have been widely used to find ligands with
pharmacological and analytical uses, and to purify or detect proteins in complex mixtures. To improve library screening,
in the last years various strategies have been developed to avoid the selection of false positive beads and to obtain
selective ligands. Currently, there is great interest in cyclic peptides because of their resistance to enzymatic degradation
and higher selectivity compared to their linear counterparts. Lots of cyclic peptide libraries protocols have been recently
developed to facilitate hits analysis. The aim of this review is to summarize the latest applications of solid phase screening
of OBOC combinatorial peptide libraries, the improvements in the screening methods including mass spectrometry
MS/MS techniques and the strategies to synthesize OBOC cyclic peptide libraries.
Keywords: Mass Spectrometry, Tandem MS/MS, peptide, peptoid, cyclic, libraries, affinity.
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