Heliotropium subulatum is an erect or procumbent perennial herb; leaves contain foliar
trichomes and its resinous exudate is used in traditional medicine. The anti-inflammatory activity of
dichloromethane fraction and isolated flavonoids was evaluated by using carrageenan and CFA-induced
paw oedema models. Similarly, the disc diffusion and microdilution methods were used for the
assessment of antimicrobial activity. Five isolated flavonoids were investigated for their antiinflammatory
and antimicrobial activities. Eriodictyol demonstrated maximum anti-inflammatory activity (53.09%) at 30.0
mg/kg dose on 6th h and similarly, it inhibited the CFA-induced arthritis swelling (41.84%) with 30.0 mg/kg dose on 8th day
respectively. As per disc diffusion and microdilution methods used for antimicrobial activity determination, the pinocembrin
was found to be most active against Staphylococcus aureus (IZ=27±0.7 mm, 08 μg/ml dose) and Candida albicans
(IZ=17±0.9 mm; 12 μg/ml dose). These investigated results revealed that the eriodictyol and pinocembrin showed significant
anti-inflammatory and antimicrobial activities. Further studies which aimed to investigate the mechanism of action of these
isolated flavonoids in the treatment of inflammations and various types of infections have been initiated.
Keywords: Heliotropium subulatum, anti-inflammatory and antimicrobial activity, flavonoids, carrageenan and CFA models.
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