Title:Effect of Aqueous Extracts From Therapeutic Plants From Indigenous Ayurvedic System on in vitro Calcium Oxalate Crystallization in Human Urine
VOLUME: 1 ISSUE: 3
Author(s):Anita Surendra Patil, Hariprasad Madhukarrao Paikrao, Ankit Subhash Kale and Surendra Raghoba Manik
Affiliation:Lab No. 106, Plant Secondary Metabolite Lab, Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444602 (M.S) India.
Keywords:Antiurolithiatic potential, therapeutic plants, urolithiasis, urine assay.
Abstract:Urolithiasis is the most painful disorder associated with formation of stone in the
urinary system. In Indian Ayurvedic system, medicinal plants are preferred as natural drug
resources. In the present study, aqueous extracts of Bryophyllum pinnatum (Lam.), Tribulus
terrestris (L.), Phyllanthus niruri (L.), Abutilon indicum (L.) and tri-sodium citrates were
used to investigate their antilithiatic potential for in vitro crystallization of calcium oxalate
crystals (CaOx) in the urine assay. With this, we conclude that presence of T. terrestris extract showed
the highest reduction in CaOx crystal size (2.70±0.41 μm2) and number (4.33±0.33) with the lowest IC50
(0.264 mg/ml) as compared to other plants in human urine (pH 6.0) with the minimum number of Calcium
oxalate dihydrate (COD) 5.66±0.66. The experiment confirms the potential of therapeutic plant extracts in
dissolving the sharp ends of Calcium oxalate monohydrate crystals (COM) making them oval to circular and
facilitating their easy exit through urinary tract.
