This paper presents deoxyribonucleic acid (DNA) detection in an optical system by utilizing
a multilayer polydimethylsiloxane (PDMS) microfluidic chamber through ultraviolet (UV) absorption
process. Nucleic acids have high absorption in the UV range between 240 to 275 nm, and this property
adapts to this work since DNA induces a significant absorbance change specifically at 260 nm. The
multilayer PDMS microfluidic was produced by a spin coat process on the SU-8 mold. An optical system
was developed to measure the change in light intensity over 500 µs, and the absorbance of the
DNA samples were derived. The optical transmission properties of various samples with different
thicknesses of PDMS are also investigated under UV light illumination. This characterization is to prove that the multilayer
PDMS microfluidic is suitable as the device for DNA detection since the thickness is less than 1000 µm and having
a higher percentage of the optical transmission. In DNA detection, significant experimental results were achieved, with
the absorbance coefficient averaged between 0.07 and 0.09 a.u.
Keywords: DNA detection, MEMS fabrication, multilayer PDMS microfluidic, optical system.
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