A polyelectrolyte is a polymer composed of repeating units of an electrolyte group that enables reversible
complex formation with proteins in aqueous solutions. This review introduces “wrap-and-strip” technology
of protein–polyelectrolyte complex (PPC) by noncovalent interaction. Storage: protein is stabilized
against physical and chemical stresses. Enrichment: precipitation through PPC can be used as an enrichment
method without irreversible unfolding. Catalytic activity switch: a complementary charged pair of polyelectrolytes
functions as a reversible enzyme activity switch. Hyperactivation: a specific combination of a polyelectrolyte
and substrate enhances enzyme activity by one order of magnitude compared with an enzyme alone.
Stabilization: PPC increases protein stability against chemical and physical stresses, such as covalently modified
polyethylene glycosylated protein. Simple PPC-based technology can expand the applicable fields of
soluble proteins in aqueous solutions.
Keywords: Antibody, enzyme, enrichment, polyelectrolyte, enzyme activity, aggregation, precipitation.
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