Current Medicinal Chemistry

Atta-ur-Rahman, FRS
Honorary Life Fellow
Kings College
University of Cambridge
Cambridge
UK

Back

Translate in Chinese

Wrap-and-Strip Technology of Protein–Polyelectrolyte Complex for Biomedical Application

Author(s): Kentaro Shiraki, Takaaki Kurinomaru, Shunsuke Tomita.

Abstract:

A polyelectrolyte is a polymer composed of repeating units of an electrolyte group that enables reversible complex formation with proteins in aqueous solutions. This review introduces “wrap-and-strip” technology of protein–polyelectrolyte complex (PPC) by noncovalent interaction. Storage: protein is stabilized against physical and chemical stresses. Enrichment: precipitation through PPC can be used as an enrichment method without irreversible unfolding. Catalytic activity switch: a complementary charged pair of polyelectrolytes functions as a reversible enzyme activity switch. Hyperactivation: a specific combination of a polyelectrolyte and substrate enhances enzyme activity by one order of magnitude compared with an enzyme alone. Stabilization: PPC increases protein stability against chemical and physical stresses, such as covalently modified polyethylene glycosylated protein. Simple PPC-based technology can expand the applicable fields of soluble proteins in aqueous solutions.

Keywords: Antibody, enzyme, enrichment, polyelectrolyte, enzyme activity, aggregation, precipitation.

Order Reprints Order Eprints Rights & PermissionsPrintExport

Article Details

VOLUME: 23
ISSUE: 3
Year: 2016
Page: [276 - 289]
Pages: 14
DOI: 10.2174/0929867323666151127201126
Price: $58