Title:The Preparation of Multifunctional Fluorescent Drug-loaded Hollow Gold Nanosphere
VOLUME: 12 ISSUE: 4
Author(s):Jie An, Ling-Yu Bai, Xiao-Shuai Zhang, Zhi-Ya Ma, Bo Liu and Yuan-Di Zhao
Affiliation:Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, the Department of Biomedical Engineering, Faculty of College of Life Science and Technology, Huazhong University of Science and Technology, P.O. Box: 430074, Wuhan, P.R. China.
Keywords:Fluorescent dye, hollow gold nanosphere, multi-modal imaging, photothermal therapy.
Abstract:Background: The present study has made great progress in multifunctional nanomaterials,
which have great potential in cancer diagnosis and therapy.
Methods: In this paper, we proposed a new multifunctional nanocomposite (HGN@RhB-BSA/DOX)
which bovine serum albumin (BSA) and doxorubicin (DOX) were modified onto hollow gold nanosphere
(HGN) by the electrostatic interaction, and fluorescent dye Rhodamine B (RhB) was combined
with bovine serum albumin (BSA) by coupling agent 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride
(EDC)/N-hydroxysuccinimide sodium salt (Sulfo-NHS).
Results: Based on absorption spectrometer, fluorescence spectrometer, and ZetaSizer analyzer, the nanocomposite had
strong surface plasmon resonance (SPR) absorption near 800 nm and revealed the success of RhB, BSA, and DOX were bound
onto HGN. The significant rising of temperature was observed when multifunctional fluorescent drug-loaded nanoparticle
were irradiated with near-infrared (NIR) light.
Conclusion: We conclude that the nanocomposite presents a promising for photothermal therapy, fluorescence imaging and
drug delivery.
