The effect of alkanes, viz. n-hexane, n-heptane and n-octane on the extracellular proteome
and secretome of a solvent tolerant Pseudomonas aeruginosa has been described. Superoxide dismutase,
GroEL, A-type flagellin and the porin OprF were altered significantly. The presence of these proteins
in the extracellular proteome of solvent-grown cells indicates their role in the cellular adaptation
of the strain to a high alkane environment. Up regulation of superoxide dismutase indicates creation of
oxidative stress for the bacterial cell in an alkane-rich environment. This secretome analysis is useful
for gaining a better understanding of the solvent mediated oxidative stress and the role of superoxide
dismutase and GroEL expression as possible markers of solvent tolerance.
Keywords: Elastase, endopeptidase, OprF, GroEL, superoxide dismutase, solvent tolerance.
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