Influence of N-glycans on Expression of Cell Wall Remodeling Related Genes in Paracoccidioides brasiliensis Yeast Cells

Author(s): Fausto Almeida, Amanda Cristina Campos Antoniêto, André Moreira Pessoni, Valdirene Neves Monteiro, Ana Claudia Paiva Alegre-Maller, Laurine Lacerda Pigosso, Maristela Pereira, Célia Maria de Almeida Soares, Maria Cristina Roque-Barreira.

Journal Name: Current Genomics

Volume 17 , Issue 2 , 2016

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Abstract:

Paracoccidioidomycosis is the most prevalent systemic mycosis in Latin America. It is caused by the temperature- dependent dimorphic fungus Paracoccidioides brasiliensis. The P. brasiliensis cell wall is a dynamic outer structure, composed of a network of glycoproteins and polysaccharides, such as chitin, glucan and N-glycosylated proteins. These glycoproteins can interact with the host to affect infection rates, and are known to perform other functions. We inhibited N-linked glycosylation using tunicamycin (TM), and then evaluated the expression of P. brasiliensis genes related to cell wall remodeling. Our results suggest that cell wall synthesis related genes, such as β-1,3-glucanosyltransferase (PbGEL3), 1,3-β-D-glucan synthase (PbFKS1), and α-1,4-amylase (PbAMY), as well as cell wall degrading related genes, such as Nacetyl- β-D-glucosaminidase (PbNAG1), α-1,3-glucanase (PbAGN), and β-1,3-glucanase (PbBGN1 and PbBGN2), have their expression increased by the N-glycosylation inhibition, as detected by qRT-PCR. The observed increases in gene expression levels reveal possible compensatory mechanisms for diminished enzyme activity due to the lack of glycosylation caused by TM.

Keywords: N-glycan, Paracoccidioides brasiliensis, Cell wall, Fungal cell.

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Article Details

VOLUME: 17
ISSUE: 2
Year: 2016
Page: [112 - 118]
Pages: 7
DOI: 10.2174/1389202917666151116212705
Price: $58

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