Curcumin as an antioxidative agent which has been widely used medicinally in India and
China. However, rapid metabolism coupled with the instability of curcumin under physiological conditions
has greatly limited its applications in vivo. In the present study, a thermosensitive hydrogel
with high payload of curcumin was developed by using a co-precipitation method, and its reversion of
oxidative stress in Neuro-2a cells was investigated. With an increase in drug loading capacity, the solgel
transition temperature of the thermosensitive hydrogel decreased accordingly. The stability of curcumin
in phosphate-buffered saline (PBS; pH=7.4) was greatly improved by encapsulation in the
thermosensitive hydrogel, as indicated by an in vitro degradation test. An in vitro release study showed that the encapsulated
curcumin was rapidly released from the hydrogel within 6 h. A curcumin/F-127 aqueous solution under the threshold
concentration of 4μg/mL was non-toxic against Neuro-2a cells after 24-h incubation. A MitoSOX assay indicated that the
developed curcumin formulation could attenuate the oxidative damage induced by H2O2 as compared to that of the H2O2
group. All these results suggested that the developed curcumin/thermosensitive hydrogel might have great potential application
in the reversion of oxidative stress after traumatic brain injury.
Keywords: Curcumin hydrogel, in vitro release, oxidative stress.
Rights & PermissionsPrintExport