Comparative Protein Profiling of Intraphagosomal Expressed Proteins of Mycobacterium bovis BCG

Author(s): Neelja Singhal, Manish Kumar, Divakar Sharma, Deepa Bisht.

Journal Name: Protein & Peptide Letters

Volume 23 , Issue 1 , 2016

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Abstract:

BCG, the only available vaccine against tuberculosis affords a variable protection which wanes with time. In this study we have analyzed and compared the proteins which are expressed differentially during broth-culture and intraphagosomal growth of M.bovis BCG. Eight proteins which showed increased expression during the intraphagosomal growth were identified by MALDI-TOF/MS. These were - a precursor of alanine and proline-rich secreted protein apa, isoforms of malate dehydrogenase, large subunit alpha (Alpha-ETF) of electron transfer flavoprotein, immunogenic protein MPB64 precursor, UPF0036 protein, and two proteins with unknown function. Based on these findings we speculate that higher expression of these proteins has a probable role in intracellular survival, adaptation and/or immunoprotective effect of BCG. Further, these proteins might also be used as gene expression markers for endosome trafficking events of BCG.

Keywords: Intraphagosomal, MALDI-TOF, M. bovis BCG, proteomics, two-dimensional gel electrophoresis, vaccine.

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Article Details

VOLUME: 23
ISSUE: 1
Year: 2016
Page: [51 - 54]
Pages: 4
DOI: 10.2174/0929866523666151106123121

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