Objective: To study the effect of exercise preconditioning on adenosine 5’triphosphate
(ATP) metabolism in red blood cells and cardiovascular protection against injury induced by
isoproterenol in vivo.
Methods: Male Sprague Dawley rats (SDR) were each exercised on a treadmill for 15 minutes at 10
m/min and 10% grade (n = 7) (LowEx), or 14 m/min and 22% grade (n = 8) (VigEx). Two hours after
the exercise, each rat received a single dose of isoproterenol (30 mg/kg) by subcutaneous (sc)
injection. Two separate groups of SDR were used as control: One received no exercise (n = 10) (NoEx) and the other
received no exercise and no isoproterenol (n = 11) (NoIso). Serial blood samples were collected over 5 hours for
measurement of ATP and its catabolites by a validated HPLC. Hemodynamic recording was collected continuously for
the duration of the experiment. Data were analysed using ANOVA and t-tests and difference considered significant at
p < 0.05.
Results: Exercise pre-conditioning (both LowEx and VigEx) reduced mortality after isoproterenol from 50% to < 30%
(p > 0.05). It attenuated the rebound in blood pressure significantly (p < 0.05 between NoEx vs VigEx), attenuated the
increase of RBC adenosine 5’-monophosphate (AMP) concentrations induced by isoproterenol, and also decreased the
breakdown of ATP to AMP in the RBC ( p < 0.05 vs NoEx).
Conclusion: Exercise pre-conditioning decreased the blood pressure rebound and also breakdown of ATP in RBC after
isoproterenol which may be exploited further as a drug target for cardiovascular protection and prevention.