Ofloxacin (OFL) loaded poly(ε-caprolactone) (PCL) and PCL: poly(butylene succinate)
PBS fibers as a drug delivery system in the treatment of ocular infections were prepared by electrospinning.
In particular, the effect of some formulation variables including polymer:drug ratio (9:1, 8:2
and 7:3 w/w), solvent systems like dichloromethane (DCM), N,N-dimethylformamide (DMF), N,Ndimethylacetamide
(DMAc) and dimethylsulfoxide (DMSO), polymer blends of PCL:PBS at 80:20,
60:40 and 40:60 ratios on fiber morphology, fiber size were investigated. The morphology and diameter
of the electrospun fibers were investigated by scanning electron microscopy (SEM) images also the thermal properties
were evaluated by differential scanning calorimetry (DSC). The drug release behaviour from fibers and in vitro antibacterial
activity were also studied. It was noticed that the average fiber diameter decreased with decreasing polymer amount in
initial composition meanwhile the release of drug increased with increasing amount of drug in formulations. Solvent system
of DCM:DMF at 80:20 ratio improved fiber morphology and resulted in a reduction in fiber diameter. It was found
that smooth surface, flexible fibers with uniform morphology were obtained with 80:20 ratio of PCL:PBS compositions.
All fibers showed a burst release of OFL. The initial amount of the released OFL was found to vary as a function of
PCL:OFL ratio and polymer composition in the fiber. The microbiological activity of optimized formulation was evaluated
using P. aeruginosa, S. epidermidis, S. Aureus and E. coli strains and the results of this study clearly demonstrated
that freely released OFL from fibers inhibited the growth of the tested bacteria. The process of electrospinning had no adverse
effect on the activity of incorporated drug in fibers.