Micro-flow Immunosensor Based on Thin-film Interdigitated Gold Array Microelectrodes for Cancer Biomarker Detection

Author(s): Andrea Ravalli, Luca Lozzi, Giovanna Marrazza.

Journal Name: Current Drug Delivery

Volume 13 , Issue 3 , 2016

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Abstract:

In this paper, we reported the development of a micro-flow label-free impedimetric biosensor based on the use of thin-film interdigitated gold array microelectrodes (IDA) for the detection of carbohydrate antigen 125 (CA125). The immunosensor is developed through the electropolymerization of anthranilic acid (AA) on the surface of IDA electrodes followed by the covalent attachment of anti-CA125 monoclonal antibody. CA125 protein affinity reaction was then evaluated by means of electrochemical impedance spectroscopy (EIS). The sensor was characterized by electrochemical techniques and scanning electron microscopy (SEM). Using the optimized experimental conditions, the developed immunosensor showed a good analytical performance for CA125 detection from 0 to 100 U/mL with estimated limit of detection (LOD = 3Sblank/Slope) of 7 U/mL.

Keywords: Antrhanilic acid, cancer antigen 125, electrochemical impedance spectroscopy, thin-film interdigitated gold array microelectrodes (IDA).

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Article Details

VOLUME: 13
ISSUE: 3
Year: 2016
Page: [400 - 408]
Pages: 9
DOI: 10.2174/1567201812666151029101217
Price: $58

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