Cyclic Peptide Containing Hydrophobic and Positively Charged Residues as a Drug Delivery System for Curcumin

Author(s): Amir Nasrolahi Shirazi, Naglaa Salem El-Sayed, Rakesh Kumar Tiwari, Kathy Tavakoli, Keykavous Parang.

Journal Name: Current Drug Delivery

Volume 13 , Issue 3 , 2016

Submit Manuscript
Submit Proposal

Graphical Abstract:


Abstract:

Due to the low water solubility and hydrophobic nature of curcumin, an efficient cellular uptake is critical for its biological activity. We have previously developed a number of homochiral L-cyclic peptides containing arginine and tryptophan as cell-penetrating peptides. Among the synthesized peptides, [WR]5 containing five arginine and five tryptophan residues was found to be the most efficient one. Here, we have compared the application of [WR]5 to improve the intracellular uptake of curcumin by using both peptide-curcumin conjugate and physical mixture (peptide + curcumin) strategies. Flow cytometry results showed that the intracellular uptake of curcumin (50 μM) was enhanced through the physical mixing with [WR]5 by 5.7 folds compared to that of curcumin alone in human leukemia (CCRFCEM) cells after 3 h. When [WR]5 was conjugated with curcumin, the intracellular uptake was enhanced by 4 fold. These data suggest that the physical mixture can work more efficiently in enhancing the cellular delivery of curcumin. Furthermore, the antiproliferative activity of curcumin was enhanced by 20% and ∼13% through the physical mixture and the conjugate, respectively, in CCRF-CEM cells after 72 h.

Keywords: Antiproliferative activity, cyclic peptides, curcumin, drug delivery systems.

Rights & PermissionsPrintExport Cite as


Article Details

VOLUME: 13
ISSUE: 3
Year: 2016
Page: [409 - 417]
Pages: 9
DOI: 10.2174/1567201812666151029101102
Price: $58

Article Metrics

PDF: 21
HTML: 2