Diarrheal diseases represent a major health problem in developing countries. Several viruses and bacterial
agents, such as Enterotoxigenic Escherichia coli (ETEC) and Enterohemorrhagic Escherichia coli (EHEC) are responsible
for human enteric infections.
In humans, EHEC infections result in bloody or non-bloody diarrhea, which may be complicated by haemorrhagic colitis
and haemolytic uraemic syndrome (HUS). Infection by ETEC is accompanied by a non inflammatory watery diarrhea. E.
coli follows a common strategy of infection: colonization on a mucosal site, evasion of host defenses, multiplication, and
host damage. Intimin, Stx, Lt and Cfa proteins are the virulence factors expressed by these strains. Antibiotic treatment is
generally not recommended for most cases of diarrhea, since antibiotic usage may lead to antibiotic resistance in ETEC
and may also change the intestinal flora. We hypothesized that the chimeric forms of these effectors as vaccine candidates
would reduce the colonization of bacteria. This study is based on an in silico analysis of chimeric protein structure and its
stability and solubility. The secondary and tertiary structures of selected domains were also predicted. Moreover, T and B
cell epitopes were mapped. Protein structure Prediction showed that each domain of antigen was separated completely
also stable for recombinant expression. We believe that this chimeric vaccine candidate is effective for prevention of bacteria
caused diarrheal diseases.