The demand for novel effective antioxidant-based drugs has led to the evaluation of the antioxidant potential in several molecules derived from natural resources. In the current work, in vitro antioxidants of methnol extract of Pinus nigra resin (PNR) from Turkey were analyzed. The antioxidant activity of methanol extract of Pinus nigra resin was evaluated using different antioxidant assays such as total antioxidant activity, DPPH free radical scavenging activity, ferrous ions (Fe2+) chelating activity, reducing power, hydrogen peroxide (H2O2) scavenging activity, superoxide anion radical (O2·-) scavenging activity and ABTS·+ scavenging activity. In addition, the amounts of total phenolics and flavonoids were also determined. The extract showed 81% inhibition on peroxidation of linoleic acid emulsion. Like metal chelating, the reducing power, hydrogen proxide scavenging and ABTS·+ scavenging activity of PNR were depending on concentration and increased with the increasing concentration of PNR. Our results indicated that the Pinus nigra resin showed the potential to be used as a natural antioxidant.
Keywords: ABTS, antioxidant activity, DPPH, metal chelating, Pinus nigra resin, reducing power.
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