Abstract
Azo dyes form substantial industrial pollutants owing to their poor biodegradation capacity. Present study identifies azo dye detoxifying strain of bacteria from waste water near textile industries. In order to identify the azo dye degrading strain, 16S rRNA gene was sequenced from the pure bacterial culture obtained from the samples collected from textile industry area at Erode in Tamil Nadu state of India. Two novel azo dye degrading bacteria were found out of 60 samples investigated, which were respectively named Sphingomonas sp strain EMBS022 and Sphingomonas sp strain EMBS023. Isolates of Sphingomonas sp strains - EMBS022 and EMBS023 were deposited in GenBank with accession numbers ‘KF951596’ & ‘KF951597’respectively. UNAFOLD and RNA fold web servers were employed to predict the secondary structure of 16s RNA of these strains. Free energy estimate for secondary structures of 16s rRNA of strains EMBS022 and EMBS023 were ΔG = -159.00 kcal/mol and ΔG = -159.90 kcal/mol, confirming the structures to be considerably stable.
Keywords: Azo dyes degrading bacteria, Sphingomonas species, Sphingomonas sp strain EMBS022 and EMBS023, 16S rRNA gene sequencing.
Current Bioinformatics
Title:Identification of Azo Dye Degrading Sphingomonas Strain EMBS022 and EMBS023 Using 16S rRNA Gene Sequencing
Volume: 10 Issue: 5
Author(s): Anuroopa G. Nadh, Uday Raj Akare, Priya Sharma, Pratibha Yadav, Srinivas Bandaru, Anuraj Nayarisseri and Achuthsankar S. Nair
Affiliation:
Keywords: Azo dyes degrading bacteria, Sphingomonas species, Sphingomonas sp strain EMBS022 and EMBS023, 16S rRNA gene sequencing.
Abstract: Azo dyes form substantial industrial pollutants owing to their poor biodegradation capacity. Present study identifies azo dye detoxifying strain of bacteria from waste water near textile industries. In order to identify the azo dye degrading strain, 16S rRNA gene was sequenced from the pure bacterial culture obtained from the samples collected from textile industry area at Erode in Tamil Nadu state of India. Two novel azo dye degrading bacteria were found out of 60 samples investigated, which were respectively named Sphingomonas sp strain EMBS022 and Sphingomonas sp strain EMBS023. Isolates of Sphingomonas sp strains - EMBS022 and EMBS023 were deposited in GenBank with accession numbers ‘KF951596’ & ‘KF951597’respectively. UNAFOLD and RNA fold web servers were employed to predict the secondary structure of 16s RNA of these strains. Free energy estimate for secondary structures of 16s rRNA of strains EMBS022 and EMBS023 were ΔG = -159.00 kcal/mol and ΔG = -159.90 kcal/mol, confirming the structures to be considerably stable.
Export Options
About this article
Cite this article as:
G. Nadh Anuroopa, Raj Akare Uday, Sharma Priya, Yadav Pratibha, Bandaru Srinivas, Nayarisseri Anuraj and S. Nair Achuthsankar, Identification of Azo Dye Degrading Sphingomonas Strain EMBS022 and EMBS023 Using 16S rRNA Gene Sequencing, Current Bioinformatics 2015; 10 (5) . https://dx.doi.org/10.2174/1574893610666151008012312
DOI https://dx.doi.org/10.2174/1574893610666151008012312 |
Print ISSN 1574-8936 |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-392X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Tumor-Induced Metabolism and T Cells Located in Tumor Environment
Current Cancer Drug Targets <i>Ajuga</i> L.: A Systematic Review on Chemical Composition, Phytopharmacological and Biological Potential
Current Bioactive Compounds Topical Therapy for Actinic Keratoses: Current and Evolving Therapies
Reviews on Recent Clinical Trials Astrocytes as a 5-HT2B-Mediated SERT-Independent SSRI Target, Slowly Altering Depression-Associated Genes and Function
Current Signal Transduction Therapy Growth Responses Following a Single Intra-Muscular hGH Plasmid Administration Compared to Daily Injections of hGH in Dwarf Mice
Current Gene Therapy Base Excision Repair: Contribution to Tumorigenesis and Target in Anticancer Treatment Paradigms
Current Medicinal Chemistry Human Defensins: Synthesis and Structural Properties
Current Pharmaceutical Design Inflammation as a Therapeutic Target in Acute Ischemic Stroke Treatment
Current Topics in Medicinal Chemistry FOXO1: A Potential Target for Human Diseases
Current Drug Targets Restoring TRAIL Induced Apoptosis Using Naturopathy. Hercules Joins Hand with Nature to Triumph Over Lernaean Hydra
Current Genomics In vitro Propagation of Cichorium intybus L. and Quantification of Enhanced Secondary Metabolite (Esculin)
Recent Patents on Biotechnology TNF-α Induction by Nickel Compounds is Specific Through ERKs/AP-1- Dependent Pathway in Human Bronchial Epithelial Cells
Current Cancer Drug Targets Integrins: Novel Therapeutic Targets for Cardiovascular Diseases
Cardiovascular & Hematological Agents in Medicinal Chemistry Colon Cancer Therapy: Recent Developments in Nanomedicine to Improve the Efficacy of Conventional Chemotherapeutic Drugs
Anti-Cancer Agents in Medicinal Chemistry CXCL8 as a Potential Therapeutic Target for HIV-Associated Neurocognitive Disorders
Current Drug Targets Establishing Genomic/Transcriptomic Links Between Alzheimer’s Disease and Type 2 Diabetes Mellitus by Meta-Analysis Approach
CNS & Neurological Disorders - Drug Targets Effects of Extracellular Matrix and Integrin Interactions on Airway Smooth Muscle Phenotype and Function: It Takes Two to Tango!
Current Respiratory Medicine Reviews Interconnection of Estrogen/Testosterone Metabolism and Mevalonate Pathway in Breast and Prostate Cancers
Current Molecular Pharmacology Characterization of Particulate Drug Delivery Systems for Oral Delivery of Peptide and Protein Drugs
Current Pharmaceutical Design Natural Compounds with Proteasome Inhibitory Activity for Cancer Prevention and Treatment
Current Protein & Peptide Science