Functional heterogeneity as reflected by topological parameters in a classical protein molecular model: t4 phage lysozyme.

Author(s): Lisa Beatrice Caruso, Alessandro Giuliani, Alfredo Colosimo.

Journal Name: Current Protein & Peptide Science

Volume 17 , Issue 1 , 2016

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Abstract:

A systematic comparison with the Wild-Type (WT) of one-point mutants of bacteriophage T4 lysozyme was carried out using as difference markers the topological parameters of the protein contact networks corresponding to each crystallographic structure. The investigation concerned changes at the resolution level of single residue along the protein sequence. The results were correlated with (reported) changes in functional properties and (observed) changes in the information provided by the energy dissipation algorithm of the “Turbine” software simulation tool. The critical factor leading to significant difference among mutants and WT is in most cases associated to the sensitivity towards mutation of relatively short windows in the amino acidic sequence not necessarily contiguous to the active site.

Keywords: Bacteriophage T4, lysozyme, network analysis, protein structure.

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Article Details

VOLUME: 17
ISSUE: 1
Year: 2016
Page: [52 - 61]
Pages: 10
DOI: 10.2174/1389203716666150923103629

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