Melanin uncontrollable accumulation is a serious social problem to not only women, but also men, and causes
pigment over-expression disorders such as freckles, melasma or pigmented acne scars. The synergism is used widely in
medication, and the effectiveness makes the drug applications more valuable. Within this experiment, three well-known
compounds were chosen: kojic acid, 1-phenyl-2-thiourea (PTU) and arbutin, and they were combined individually with
our substance linderanolide B, which is purified from Cinnamomum subavenium. Hence, deciphering the synergistic action
of possible whitening agents was the goal of this study. The tyrosinase activity, melanin content, and the combination
index (CI) values were observed in B16F10 cells, in addition, the consequences were detected by isobologram analysis.
We discovered that certain melanin inhibitors showed synergistic properties when they were combined together to suppress
tyrosinase activities. As a result, linderanolide B has a potential synergy on tyrosinase inhibition, and it can be used
widely in cosmetic and medication industries.