The aldol reactions catalyzed by aldolases are an important tool in the synthesis of different products or
building blocks. However, despite its advantages, aldolases, as enzymes, have some troubles as their low activity
against synthetic substrates or the low stability, doing difficult their application. There are different tools to improve
the properties of this enzyme such as directed mutagenesis, directed evolution or the immobilization methods.
The immobilization not only is able to improve the properties of the enzymes but also permits the reuse of the
catalysts. This review is focused on the immobilization of different aldolases using different methodologies.