A novel steroidal oxime (SO) was synthesized using an economic method and then characterized
by Fourier transform infrared (FTIR) and 1H nuclear magnetic resonance (NMR). When dispersed
in pure water, SO can self-assemble into a nano-scale circular structure which was verified by
dynamic light scattering (DLS) and transmission electron microscopy (TEM). The formation of SO
nano aggregates may result from its ambiphilic characteristic which has the hydrophobic cholesterolbased
backbone and the hydrophilic oxime head group. The prepared SO aggregates were then examined
for its in vitro biocompatibility using the human keratinocytes cell line (HaCaT) as a representative
skin exposure model. The biocompatibility was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assay and cell morphology observation. The results showed that the nano-scale SO aggregates did not
cause serious damage to HaCaT cells at the designed concentrations and suggested that SO could be a potential material
for preparing cosmeceutical carrier.
Keywords: Biocompatibility, nano-scale aggregates, synthesis, steroidal oxime, self-assembly.
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