Downstream Processing of Antibodies: Potential of Affinity Chromatography

Author(s): Mysore Chakrapani Lakshmi, Mudalabeedu Chandregowda Madhusudhan, Mullapudi Srinivas.

Journal Name:Current Biochemical Engineering

Volume 2 , Issue 2 , 2015

Graphical Abstract:


The increasing use of antibodies in various biotechnological and pharmaceutical applications has pushed demand to produce antibodies in larger quantities. Considerable interest has now focused on reducing manufacturing costs and reorganizing process development activities to facilitate commercialization. Hence, there is an absolute requirement for purification techniques that meet the stringent quality requirements of these therapeutic agents. Presently, a select combination of techniques are applied to purify antibodies, of which, the most frequent are salting out, affinity chromatography and ion exchange chromatography. Of these, affinity chromatography is increasingly being used as a first step in the purification of antibodies due to its high specificity. In the current review, we focus on the potential of various affinity chromatography practices with an emphasis on their power and limitations.

Keywords: Downstream processing (DSP), recombinant antibodies, monoclonal antibodies, affinity (ligand) chromatography, bio-pharma applications.

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Article Details

Year: 2015
Page: [198 - 206]
Pages: 9
DOI: 10.2174/2212711902999150806160118
Price: $58