Investigation of 5-HT2A gene expression in PBMCs of patients with allergic asthma

Author(s): Ghasem Ahangari , Somayeh Emadi Koochak , Leila Mohammadi Amirabad , Gholamreza Derkhshan Deilami .

Journal Name: Inflammation & Allergy - Drug Targets (Discontinued)

Volume 14 , Issue 1 , 2015

Abstract:

Background: Asthma is an inflammatory airway disorder in which different immune cells in the blood and lungs play a fundamental role. In asthma condition, the airway inflammation accompanied by bronchial smooth muscle spasm cause airway obstruction. A study showed that high concentration of blood serotonin is associated with the intensity and exacerbation of asthma disease. Other studies showed that a subtype of serotonin receptor called 5-Hydroxytriptamine 2A receptor (5- HT2A) can enhance T-cell blastogenesis and production of pro-inflammatory cytokines such as IFNγ. Objective: The objective of this study was to assess the level of 5-HT2A in peripheral blood mononuclear cells (PBMCs) of asthmatic patients. Methods: PBMCs were extracted from blood of 30 patients with asthma and 30 normal people. After synthesizing cDNAs from total mRNAs, real-time PCR was performed to amplify 5-HT2A and β-actin (as an internal control). The expression ratios were analyzed in patients with asthma in comparison with normal group. Results: The results indicated that gene expression is significantly increased in peripheral blood mononuclear cells (PBMCs) of asthma patients in comparison with normal group (P = 0.003). Conclusion: The results of this study can suggest designing a protocol by using of the 5-HT2A receptor expression in PBMCs as a biomarker of asthma, but this requires further studies on a larger number of patients. In addition, the potential role of this receptor in bronchoconstriction can lead us to use its antagonists as a new treatment in asthma.

Keywords: Allergic asthma, 5-HT2A> receptor, real time PCR, serotonin, serotonin receptor 2A gene.

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Article Details

VOLUME: 14
ISSUE: 1
Year: 2015
Page: [60 - 64]
Pages: 5
DOI: 10.2174/1871528114666150803152433

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