In vivo and in silico Investigation of Antidiabetic Activity of Fruit of Withania coagulans Dunal
Sudhanshu Kumar Bharti,
Neeraj Kumar Sharma,
Ashok Kumar Gupta,
This study evaluates the antidiabetic activities of methanolic extract of Withania
coagulans Dunal (Ashutosh booti) fruit (WCFE) in poloxamer-407 induced type 2 diabetic
Wistar rats. The electrochemical behaviour of WCFE with anodic peak of 1.19± 0.01V was found
similar to standards used indicating that extract is antioxidant in nature. Unlike diabetic control
rats, the WCFE treated diabetic rats presented significant amelioration of glycaemia, insulinamia
and lipid dysmetabolism, remarkable reduction of oxidative markers and improved cecal and pancreatic characteristics.
HYBRID and FRED docking were performed for 25 documented WCFE botanicals for putative action mechanism
concerning three diabetic therapeutic proteins namely PTP-1B, PPAR-γ and DPP-IV fully support the in vivo findings.
Botanicals like nicandrenone10 and Acnistin F have shown considerable interaction potential with aforesaid proteins.
Results provide pharmacological evidence of WCFE as antihyperglyceamic mediated by interaction of various botanicals
with various targets operating in diabetes mellitus.
Keywords: Cyclic voltammetry, DPP-IV, Homeostatic model assessment, Molecular docking, PPAR-γ, PTP-1B, Type 2
diabetes mellitus, Withania coagulans fruit.
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