Background: The purpose of the present study was the distribution of blaOXA-1, blaPER-1 and blaVEB
genes and the genotyping of these genes in extended-spectrum β-lactamases (ESBL) producing uropathogenic
and diarrheagenic Escherichia coli isolates. Methods: Of 432 isolates, 58 uropathogenic E. coli (UPEC) and
56 diarrheagenic E. coli (DEC) isolates were shown to produce the ESBLs. The isolates were screened to present
ESBLs genes by PCR. Pulsed-Field Gel Electrophoresis (PFGE) was used to genotype E. coli strains
which possess ESBLs genes. Results: The blaOXA-1 gene was detected in 17.24% of UPEC isolates and 1.78%
of DEC isolates. Of all the isolates studied, none were positive for the blaPER-1 and blaVEB genes. PFGE revealed
that the E. coli isolates possessed blaOXA-1 gene comprised three distinct genotypes. Conclusions: The
current study illustrated high rates of ESBL-resistant phenotypes of E. coli isolates from urine and diarrhea
samples in Iran. The blaOXA-1, blaPER-1 and blaVEB genes were found at low frequencies in studied isolates.
Keywords: Escherichia coli, extended-spectrum β-lactamases, genotyping, PFGE.
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