Herein, we describe a novel, microwave-assisted synthesis of a binding-based probe capable
of enriching proteins that bind adenine ribose derivatives (AdoRs). We employed this probe in activity-
based protein profiling studies to qualitatively assess the AdoR-binding proteome in three bacterial
cell lines from the genus Bacillus. This proof of concept experiment demonstrated a unique subset
of proteins distinctive to each species, and confirmed the efficacy of the probe tagging and subsequent
Keywords: AdoRs, binding-based probe (BBP) binding-based protein profiling (BBPP), microwave-assisted synthesis.
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