A Facile, Microwave-Assisted Synthesis of an Adenosine-Ribose Probe for Binding-Based Profiling of Nucleoside and Nucleotide-Binding Proteins

Author(s): Matthew R. Battistini , Shikha Mahajan , Daili Diaz , Lindsey N. Shaw , David J. Merkler .

Journal Name: Current Microwave Chemistry

Volume 3 , Issue 2 , 2016

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Abstract:

Herein, we describe a novel, microwave-assisted synthesis of a binding-based probe capable of enriching proteins that bind adenine ribose derivatives (AdoRs). We employed this probe in activity- based protein profiling studies to qualitatively assess the AdoR-binding proteome in three bacterial cell lines from the genus Bacillus. This proof of concept experiment demonstrated a unique subset of proteins distinctive to each species, and confirmed the efficacy of the probe tagging and subsequent enrichment.

Keywords: AdoRs, binding-based probe (BBP) binding-based protein profiling (BBPP), microwave-assisted synthesis.

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Article Details

VOLUME: 3
ISSUE: 2
Year: 2016
Page: [124 - 130]
Pages: 7
DOI: 10.2174/2213335602666150706182206
Price: $58

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