A Facile, Microwave-Assisted Synthesis of an Adenosine-Ribose Probe for Binding-Based Profiling of Nucleoside and Nucleotide-Binding Proteins

Author(s): Matthew R. Battistini, Shikha Mahajan, Daili Diaz, Lindsey N. Shaw, David J. Merkler.

Journal Name: Current Microwave Chemistry

Volume 3 , Issue 2 , 2016

Become EABM
Become Reviewer


Herein, we describe a novel, microwave-assisted synthesis of a binding-based probe capable of enriching proteins that bind adenine ribose derivatives (AdoRs). We employed this probe in activity- based protein profiling studies to qualitatively assess the AdoR-binding proteome in three bacterial cell lines from the genus Bacillus. This proof of concept experiment demonstrated a unique subset of proteins distinctive to each species, and confirmed the efficacy of the probe tagging and subsequent enrichment.

Keywords: AdoRs, binding-based probe (BBP) binding-based protein profiling (BBPP), microwave-assisted synthesis.

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2016
Page: [124 - 130]
Pages: 7
DOI: 10.2174/2213335602666150706182206
Price: $58

Article Metrics

PDF: 18