Recent Developments in Nanoparticulate Based Reverse Micellar Extraction for Downstream Processing of Biomolecules
Ram Saran Chaurasiya,
H. Umesh Hebbar,
The aim of this review paper is to highlight the recent developments in downstream processing
of biomolecules using reverse micellar extraction (RME) technique. Reverse micelles (RMs)
are a nanometer size aggregation of surfactant molecules in organic solvents. The inner core of RMs
contains an aqueous phase, which can solubilize biomolecules such as amino acids, proteins, enzymes,
and DNA. RME is a two-step purification technique, which involves solubilization of biomolecule into
RMs (forward extraction) and recovery of these biomolecules from RMs to fresh stripping phase
(back-extraction). Solubilization and the recovery of biomolecules are possible by altering processing parameters like pH
of aqueous phase, type of salt, ionic strength, type of organic solvent, surfactant type, phase contact time, operating
temperature and size of RMs. The importance of these processing conditions has been discussed in detail in this review.
Mathematical modeling of mass transfer in RME is also covered. This review highlights the application of RME in biomolecule
purification from the natural source, nanoparticle synthesis, refolding of proteins and selective separation of
biomolecules from a mixture. Apart from this, we have also discussed about the integration of RME with other
downstream processing (DSP) techniques, recent developments in innovative cost effective RME technique, recycling of
RME components and future prospects for RME.
Keywords: Nanoparticulate, reverse micellar extraction, downstream processing, surfactant, biomolecules.
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