Biogenic synthesis of metallic nanoparticles using plant materials is an emerging
field and it serves as an alternative to physical and chemical approaches. In this present work we have used a
Tribulus terrestris plant dried leaf extract for silver nanoparticles (AgNPs) synthesis. The nanoparticles (NPs)
synthesis was achieved by addition of leaf extract with aqueous silver nitrate under controlled conditions. The
formation of AgNPs was primarily confirmed by UV-Vis Spectrophotometer. The characterization of AgNPs
was done by analytical techniques such as FTIR, XRD, AFM, FESEM and EDAX spectrum. FESEM images
showed the AgNPs were spherical shaped and the particles sizes were found to be 18-47nm respectively. The
synthesized AgNPs were highly stable after three months of storage period at 37˚C. Moreover, the synthesized
AgNPs showed potent antibacterial property against clinically isolated multi-drug resistant (MDR) microorganisms.
Keywords: AFM, Biosynthesis, FESEM, MDR, Tribulus terrestris, XRD.
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