Grapevine virus A (GVA), a flexible filament of approximately 800 nm in length is composed
of capsid subunits that spontaneously assembles around a positive sense genomic RNA. In addition
to encapsidation, plant viruses capsid proteins (CPs) participate in other processes throughout infection
and GVA CP is involved in cell-to-cell translocation of the virus. A protocol was developed to
obtain low-molecular weight GVA-CP that is not prone to aggregation and spontaneous assembly and
this was characterized by circular dichroism and dynamic light scattering. These results indicate the
suitably of GVA-CP for X-ray crystallographic and NMR studies that should lead to the elucidation of
the first three-dimensional structure of a flexible filamentous virus from the Betaflexiviridae family.
Keywords: Capsid protein, CD spectroscopy, dynamic light scattering, grapevine virus A, protein characterization, secondary.
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