Preparation and Characterization of Monomodal Grapevine Virus A Capsid Protein

Author(s): Vinícius S. Santana, Ricardo B. Mariutti, Raphael J. Eberle, Anwar Ullah, Icaro P. Caruso, Raghuvir K. Arni.

Journal Name: Protein & Peptide Letters

Volume 22 , Issue 8 , 2015

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Abstract:

Grapevine virus A (GVA), a flexible filament of approximately 800 nm in length is composed of capsid subunits that spontaneously assembles around a positive sense genomic RNA. In addition to encapsidation, plant viruses capsid proteins (CPs) participate in other processes throughout infection and GVA CP is involved in cell-to-cell translocation of the virus. A protocol was developed to obtain low-molecular weight GVA-CP that is not prone to aggregation and spontaneous assembly and this was characterized by circular dichroism and dynamic light scattering. These results indicate the suitably of GVA-CP for X-ray crystallographic and NMR studies that should lead to the elucidation of the first three-dimensional structure of a flexible filamentous virus from the Betaflexiviridae family.

Keywords: Capsid protein, CD spectroscopy, dynamic light scattering, grapevine virus A, protein characterization, secondary. structure.

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Article Details

VOLUME: 22
ISSUE: 8
Year: 2015
Page: [712 - 718]
Pages: 7
DOI: 10.2174/0929866522666150625111607
Price: $65

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