Current Biotechnology

Pabulo Henrique Rampelotto  
Center of Biotechnology and PPGBCM
Federal University of Rio Grande do Sul
Porto Alegre, RS


Rapid Estimation of Beta-Lactam Antibiotics in Biological Fluids

Author(s): Bernard Joris, Jacques Degelaen, Frank Klein, Jean-Marie Frere.

Graphical Abstract:


The Actinomadura R39 DD-peptidase is a very penicillin-sensitive enzyme. After a short time of contact with a liquid sample containing a beta-lactam antibiotic, the determination of the residual enzyme activity allows estimation of the concentration of the antibiotic in the sample. An alternative method utilizes the C-terminal domain of the Bacillus licheniformis penicillin sensor BlaR that is devoid of enzymatic activity. In this case, the protein is labelled with gold particles and the unreacted portion is captured by beta-lactam molecules covalently attached to a solid support. Both methods have been quite successful for the rapid estimation of beta-lactam antibiotics in milk.

Keywords: Beta-lactam antibiotics, penicillin-sensitive enzyme

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Article Details

Year: 2015
Page: [145 - 148]
Pages: 4
DOI: 10.2174/2211550104666150624171854
Price: $58