Current Biotechnology

Pabulo Henrique Rampelotto  
Center of Biotechnology and PPGBCM
Federal University of Rio Grande do Sul
Porto Alegre, RS
Brazil

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Analytical Methods for the Measurement of Catechol-O-Methyltransferase Activity in Animal Tissues

Author(s): Makoto Tsunoda.

Graphical Abstract:


Abstract:

Catechol-O-methyltransferase (COMT) catalyzes the transfer of a methyl group from Sadenosyl- L-methionine to catechols. Because COMT has an important role in the metabolism of catecholamines, catechol estrogens, and drugs with a catechol moiety such as L-Dopa, measurement of COMT activity in several tissues is important. Furthermore, COMT inhibitors are used clinically in the treatment of Parkinson’s disease, and activation of COMT can control high blood pressure. Hence, high-throughput screening methods for COMT inhibitors and activators are needed. In this methodological article, two analytical methods for the measurement of COMT activity are described. One is very sensitive, and the other is a rapid assay. Norepinephrine, an endogenous compound was used as the substrate, and the enzymatic product (normetanephrine) was quantified with highperformance liquid chromatography-fluorescence or chemiluminescence detection.

Keywords: Chemiluminescence, enzyme, fluorescence, HPLC, Parkinson’s disease, substrate.

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Article Details

VOLUME: 4
ISSUE: 2
Year: 2015
Page: [197 - 201]
Pages: 5
DOI: 10.2174/2211550104666150605222350
Price: $58