Effect of combination of undifferentiated bone marrow stromal cells (BMSCs) and pulsed electromagnetic
fields (PEMF) on transected sciatic nerve regeneration was assessed in rats. A 10 mm nerve segment was excised and a
vein graft was used to bridge the gap. Twenty microliter undifferentiated BMSCs (2 107 cells /mL) were administered
into the graft inBMSC group with no exposure to PEMF. In BMSC/PEMF group the whole body was exposed to PEMF
(0.3 mT, 2Hz) for 4h/day within 1-5 days. In PEMF group the transected nerve was bridged and phosphate buffered saline
was administered into the graft. In authograft group (AUTO), the transected nervesegments were reimplanted reversely
and the whole body was exposed to PEMF. The regenerated nerve fibers were studied within 12 weeks after surgery. Behavioral,
functional, electrophysiological, biomechanical, gastrocnemius muscle mass findings, morphometric indices and
immuonohistochemical reactions confirmed faster recovery of regenerated axons in BMSC/PEMF group compared to
those in the other groups (P<0.05). The use of undifferentiated BMSCs with whole body exposure to PEMF improved
functional recovery. Combination of local transplantation of in vitro bone-marrow stromal cells and pulsed electromagnetic
fields could be considered as an effective, safe and tolerable treatment for peripheral nerve repair in clinical practice.
Keywords: Nerve regeneration, sciatic, undifferentiated BMSC, PEMF, functional recovery.
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