Effect of the Bioactive Fraction of Argyreia speciosa Leaves Against Gastric Ulcer and Antioxidant Defence System in Rats
Sunil K. Jaiswal,
Ch. V. Rao,
Mukesh K. Dubey.
Object: The standardized flavonoid fraction with quercetin and kaempferol of
Argyreia speciosa (Convolvulaceae) leaves gathered from Assam (India) was used to
evaluate the ulcerprotective, antioxidant and antisecretory activities in ulcerated rats.
Materials and methods: The flavonoid enriched fraction of A. speciosa leaves (BFAS) at
dose of 50, 100 and 200 mg/kg/BW was given through oral routes, bis in die for five days for the protection
of ulcers induced by pylorus ligation, aspirin, cold restraint stress and ethanol. Estimation of in vivo
antioxidant enzyme activities were carried out in cold restraint stress induced ulcer model, whereas gastric
secretion and mucosal studies were carried out in pylorus ligation induced ulcer model. Results: A dose
dependent ulcerprotective activity was observed by BFAS, when compared with the disease control in
respective models [Pylorus ligation; 22.40-66.12% (P<0.01 to P<0.001), Aspirin; 24.70-61.17% (P<0.05 to
P<0.001), Cold-Restraint Stress; 23.34-54.18% (P<0.05 to P<0.001), Ethanol; 17.91-64.16% (P<0.01 to
P<0.001) and Ranitidine; 63.87-74.30% (P<0.01)]. The levels of antioxidant enzyme, lipid peroxidase and
superoxide dismutase were decreased, though a noteworthy increment in catalase and glutathione peroxidase
values were observed. In pylorus ligation induced ulcer model, BFAS was showed reduced gastric discharge
parameters and increased mucoprotein level with respect to ulcer control. Conclusion: The results of this
study have shown that BFAS have a significant dose dependent ulcerprotective, antioxidant and antisecretory
activity presumably by blocking the activity of proton pump, protecting from antioxidants produced during
stress induced ulcer and by enhancing glycoprotein levels.
Keywords: Antiulcer, antioxidant enzyme, antisecretory, flavonoid, gastric mucosa.
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