A new, simple, rapid, accurate and reproducible reverse phase high performance liquid
chromatographic method with UV detection was developed and used for the estimation of abacavir
sulphate and fosamprenavir in bulk and in their pharmaceutical dosage forms. High performance
liquid chromatography was carried out on a Waters Spherisorb ODSI (250x4.6mm with 5 μm
particle size) column with a flow rate 1.0 mL.min-1 using a mobile phase consisting of methanol:
phosphate buffer (80:20, v/v) containing 15mM phosphoric acid at pH 6.2. The detection was carried
out at 275 nm and the run time was 5 min. For the sensitive determination of abacavir, fosamprenavir was used as an
internal standard and for determination of fosamprenavir vice versa. Using these conditions, both antiviral drugs were
analyzed simultaneously with the same method and same chromatographic conditions. The linearity was found to be in
the range of 0.25-75 μg.min-1 and 0.50-75 μg.min-1 for abacavir and fosamprenavir, respectively. The retention times for
fosamprenavir and abacavir were 2.48 and 4.04 min, respectively. The proposed method was validated according to ICH
and USP guidelines and application of the method to tablet dosage forms were also performed.
Keywords: Abacavir, antiviral drug, determination, fosamprenavir, pharmaceuticals, reverse phase liquid chromatography.
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