Environmental Stress Tolerance Engineering by Modification of Cell Surface and Transcription Factor in Saccharomyces cerevisiae
In bioindustrial processes, organisms are subjected to a variety of stresses such as heavy metal ions, organic
solvents, pH and high temperature. These stresses diminish or abolish their biological activities and cell growth, resulting
in reduced efficiency of bioproduction. To maintain high bioproduction rates, stress-tolerant organisms that can grow and
produce useful materials under stressful conditions are required. Recent studies have demonstrated that cell surface
engineering and modification of transcription factors can improve the stress tolerance of organisms, especially the yeast
Saccharomyces cerevisiae. The cell surface is in direct contact with the external environment and plays an essential role in
stress responses. Therefore, artificial modification of the cell surface is an attractive way to improve tolerance against
environmental stresses. In addition, transcription factors activate genes involved in a wide variety of stress response
functions, including signal transduction, transcriptional control, transportation, and scavenging of toxic compounds.
Mutations in transcription factors likely cause dynamic changes in the cellular system and can greatly improve stress
tolerance. In this review, we summarize recent studies demonstrating improvement of tolerance to heavy metal ions, acid,
organic solvents, and heat by modification of the cell surface and transcription factors of S. cerevisiae.
Keywords: Bioproduction, cell surface engineering, master regulator, Saccharomyces cerevisiae, stress tolerance, transcription
Rights & PermissionsPrintExport