Voltammetric Sensing of Uremic Toxin Indoxyl Sulfate Using High Performance Disposable Screen-Printed Graphene Electrode

Author(s): Hayati Filik , Asiye A. Avan , Sevda Aydar .

Journal Name: Current Pharmaceutical Analysis

Volume 12 , Issue 1 , 2016

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Abstract:

We focused on a rapid method for the assay of protein unbound indoxyl sulfate (IS) in urine samples by square-wave voltammetry. A sensor based on the electroactivity of commercial graphene screen-printed electrode (GR-SPE) for sensitive estimation of uremic toxin indoxyl sulfate was presented. The behaviors of uremic solute indoxyl sulfate on GR-SP electrodes were explored by cyclic and square-wave voltammetry. The outcomes displayed that the GR-SP electrode exposed perfect electroactivity to uremic toxin indoxyl sulfate and the oxidation of uremic solute IS is a multi-step and irreversible process. The process is pH dependent and takes place with the forging of a main oxidation product that is adsorbed on the GR-SP electrode surface. The calibration curve was achieved in the IS concentration range of 0.5–80 µM and the limit of detection was calculated to be 0.064 µM at a signal-to-noise ratio of 3. The use of the commercially disposable GR-SPE in human serum and urine sample assays obtained satisfactory results, which made it a promising alternative in routine sensing applications.

Keywords: Biomolecule, electrochemical sensing, indoxyl sulfate, serum, uremic toxin, urine analysis.

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Article Details

VOLUME: 12
ISSUE: 1
Year: 2016
Page: [36 - 42]
Pages: 7
DOI: 10.2174/1573412911666150520000605
Price: $58

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