Background/Purpose: Medicinal plant extracts with potent antimicrobial activity are potentially
useful sources of new and novel antimicrobial agents. We investigated the extracts from the stem
bark of Macaranga rosea antimicrobial activity. Methods: Cold extraction procedures were used to obtain
extracts followed by standard qualitative assays for phytochemicals. Agar well diffusion and macrobroth
dilution assays were used to determine antimicrobial effects of the extracts. Thin layer and column
chromatography were used to further separate the chemical entities for further clues on the active
constituents. Results: The Methanolic extracts provided the greatest diversity of phytochemicals including
reducing sugars, anthraquinones, Phlobatannins, glycosides, steroids, tannins, saponins and alkaloids. Ethanol extracts
were the most effective against Staphylococcus aureus and Pseudomonas aeruginosa whilst methanolic extracts
were more effective against Candida albicans. The MIC and MBC values were lowest with the methanolic extracts for S.
aureus with values of 3.12 and 6.25 mg/ml for MIC and MBC respectively. For P. aeruginosa, the ethanolic extract gave
the best MIC and MBC values of 1.56 and 3.12 mg/ml respectively. For C. albicans, the best MIC and MFC values were
obtained from methanolic extracts at 0.78 and 3.12 mg/ml respectively. Fractions of the methanolic extracts were active
against Pseudomonas aeruginosa and Candida albicans but not S. aureus at 250µg/ml. Conclusion: This study demonstrated
the phytochemical richness and wide spectrum of antimicrobial activity of Macaranga rosea. The plant is currently
endangered and this study appears to be the first documented on its antimicrobial properties.
Keywords: Anti-Pseudomonas, fungicidal, Macaranga rosea, phytochemical.
Rights & PermissionsPrintExport