A Derivative Method with Free Radical Oxidation to Predict Resveratrol Metabolites by Tandem Mass Spectrometry

Author(s): Wangta Liu, Yow-Ling Shiue, Yi-Reng Lin, Hugo You-Hsien Lin, Shih-Shin Liang.

Journal Name: Current Analytical Chemistry

Volume 11 , Issue 4 , 2015

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Abstract:

In this study, we demonstrated an oxidative method with free radical to generate 3,5,4-trihydroxy-transstilbene (trans-resveratrol) metabolites and detect sequentially by an autosampler coupling with liquid chromatography electrospray ionization tandem mass spectrometer (LC-ESI–MS/MS). In this oxidative method, the free radical initiator, ammonium persulfate (APS), was placed in a sample bottle containing resveratrol to produce oxidative derivatives, and the reaction progress was tracked by autosampler sequencing. Resveratrol, a natural product with purported cancer preventative qualities, produces metabolites including dihydroresveratrol, 3,4-dihydroxy-trans-stilbene, lunularin, resveratrol monosulfate, and dihydroresveratrol monosulfate by free radical oxidation. Using APS free radical, the concentrations of resveratrol derivatives differ as a function of time. Besides simple, convenient and time- and labor saving, the advantages of free radical oxidative method of its in situ generation of oxidative derivatives followed by LC-ESI–MS/MS can be utilized to evaluate different metabolites in various conditions.

Keywords: Free radical, metabolite, resveratrol, ammonium persulfate (APS), liquid chromatography tandem mass spectrometry (LC–MS/MS), multiple reaction monitoring (MRM).

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Article Details

VOLUME: 11
ISSUE: 4
Year: 2015
Page: [300 - 306]
Pages: 7
DOI: 10.2174/1573411011666150515233817

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