Proteomic Analysis of the Resistant Responses of Two Vitis amurensis Cultivars to Plasmopara viticola Infections
Leaves of Vitis amurensis ‘Shuangyou’ (susceptible) and ‘Shuanghong’ (resistant) were inoculated with Plasmopara
viticola, the causal agent of downy mildew in grapevine. Key proteins regulated in the response to downy mildew
infection were analyzed by differential proteomics and MALDI-TOF/TOF. The leaf proteome changed differentially between
the two cultivars following injection by P. viticola. More than 300 protein spots were detected, among which nine
differentially expressed proteins were identified. The functions of the identified proteins comprised secondary metabolism,
structure, photosynthesis, energy production, and resistance. In the resistant cultivar, photosynthesis proteins and
ATP synthase might be down-regulated to restrict fungal growth, and the pathogenesis-related protein 10 may be upregulated
to limit the extent of spread of the fungus. Pathogenesis-related protein 10 is proposed to be a key protein in the
response of Vitis amurensis to downy mildew infection in the resistant cultivar.
Keywords: Vitis amurensis, downy mildew, differential proteomics.
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